Sheep CRP (C-Reactive Protein) ELISA Kit | ELK8302

Sheep CRP (C-Reactive Protein) ELISA Kit | ELK8302 | Gentaur UK, US & Europe Distribution

Alternative Names: C-RP; PTX1; Pentraxin-Related; Pentraxin 1

Species: sheep

Assay Type: Sandwich

Sensitivity: 35 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Reactive Protein (CRP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C-Reactive Protein (CRP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Reactive Protein (CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Reactive Protein (CRP) in the samples is then determined by comparing the OD of the samples to the standard curve.