SHISA3 Antibody | 7275

SHISA3 Antibody | 7275 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: SHISA3 antibody was raised against a 15 amino acid peptide near the center of human SHISA3 .
The immunogen is located within amino acids 120 - 170 of SHISA3.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: SHISA3 Antibody can be used for detection of SHISA3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: SHISA3 antibody is predicted to not cross-react with other SHISA protein family members.

Positive Control 1: Cat. No. 1308 - Human Small Intestine Tissue Lysate

Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 26 kDa
Observed: 28 kDa

Validation: N/A

Isoform: N/A

Purification: SHISA3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SHISA3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SHISA3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SHISA3 Antibody: hShisa3, Protein shisa-3 homolog

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SHISA3 Antibody: SHISA3 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.