Shrimp Alkaline Phosphatase, Recombinant | 9216

Shrimp Alkaline Phosphatase (SAP) is a high specific activity, heat-labile alkaline phosphatase purified from a recombinant source and originally isolated from Pandalus borealis (arctic shrimp).

9216 | Shrimp Alkaline Phosphatase Recombinant DataSheet

Biomolecule/Target: Dephosphorylation of DNA, RNA, Protein

Synonyms: SAP, rSAP

Alternates names: SAP, rSAP

Taglines: High specific activity, heat-labile alkaline phosphatase purified from a recombinant source.

NCBI Gene ID #: Ensembl: ENSMMUT00000015626

NCBI Gene Symbol: SAA

Gene Source: N/A

Accession #: P02738

Recombinant: Yes

Source: Purified from a recombinant source

Purity by SDS-PAGEs: 97%

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): 1 unit/l

Biological activity: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit is the amount of enzyme which catalyzes the hydrolysis of 1 mol of p-nitrophenyl phosphate per min in glycine buffer (pH 10.4) at 37°C.

Molecular Weight: Homodimer. Monomer is 55 kDa as determined by amino acid sequence.

Concentration: N/A

Appearance: Liquid

Physical form description: Recombinant Shrimp Alkaline Phosphatase in 25mM Tris-HCl (pH 7.5), 1mM MgCl2, 50% glycerol.

Reconstitution Instructions: Reconstitute in sterile ddHO to a concentration 100 µg/ml. This solution can then be diluted into other aqueous buffers.

Amino acid sequence: RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY