SIK1 Antibody | 6299

SIK1 Antibody | 6299 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (73%) , Mouse: (73%)

Immunogen: SIK1 antibody was raised against an 18 amino acid synthetic peptide near the center of human SIK1.
The immunogen is located within amino acids 340 - 390 of SIK1.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P

Application: SIK1 antibody can be used for detection of SIK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: Two isoforms of SIK1 are known to exist; this antibody will detect both isoforms. SIK1 antibody is predicted to not cross-react with other SIK protein family members.

Positive Control 1: Cat. No. 1308 - Human Small Intestine Tissue Lysate

Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: SIK1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SIK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SIK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SIK1 Antibody: MSK, SIK, SNF1LK, Salt-inducible kinase 1, SIK-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SIK1 Antibody: Salt-inducible kinase 1 (SIK1) , also known as SNF1LK or MSK, plays a role in histone modification and G2/M cell cycle regulation. It is a 783 amino acid protein that contains one UBA domain and belongs to the Ser/Thr protein kinase family (AMPK subfamily) . Localized to both the nucleus and the cytoplasm, SIK1 is a class II HDAC kinase that uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation.