Single Stranded DNA Binding Protein, E. coli | 9220

Single Stranded DNA Binding Protein (SSB) is a homo-tetrameric protein, which binds onto single stranded DNA (ssDNA) to coordinate replication, repair, and recombination. It possesses a N-terminal DNA binding core and a C-terminal protein-protein interacting tail. ssDNA can form secondary structures and SSB prevents their formations. SSB can be added to PCR and DNA sequencing reactions to enhance the DNA polymerases processivity by allowing them to access their substrate more easily.

9220 | Single-Stranded DNA Binding Protein DataSheet

Biomolecule/Target: N/A

Synonyms: SSB,Single- Stranded DNA Binding Protein, E. coli

Alternates names: SSB,Single- Stranded DNA Binding Protein, E. coli

Taglines: A homo-tetrameric protein, which binds onto single stranded DNA to coordinate replication, repair, and recombinatio

NCBI Gene ID #: Ensembl: ENSMMUT00000015626

NCBI Gene Symbol: SAA

Gene Source: N/A

Accession #: P02738

Recombinant: TRUE

Source: E. coli strain containing an overproducing clone of E. coli SSB protein.

Purity by SDS-PAGEs: 97%

Assay: The activity of SSB protein was determined using Gel Shift Assay. Varying amounts of SSB (150 ng to 86 g) were able to bind onto 22 fmol of M13mp18 ssDNA in a 20 l reaction volume and affect ssDNA mobility. Sample was loaded using 10x Orange DNA Loading Buffer (BV Cat# 2110-10) onto a 1% agarose Gel. Gel was electrophoresed at 3 V/cm for 5 hr.

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18.9 kDa

Concentration: Lot specific

Appearance: Liquid

Physical form description: Liquid in 50 mM Tris pH 8.0 and 50% Glycerol

Reconstitution Instructions: Reconstitute in sterile ddHO to a concentration 100 µg/ml. This solution can then be diluted into other aqueous buffers.

Amino acid sequence: RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY