SIRT3 (Human) ELISA Kit - E5009

E5009 | SIRT3 (Human) ELISA Kit DataSheet

Sirtuin3 (SIRT3) is one of the seven homologs of the yeast histone deacetylase, silent information regulator (Sir2). This NAD+-dependent deacetylase is predominantly localized in the mitochondria, and its major function to maintain homeostasis in mitochondria under stress conditions. SIRT3 is highly expressed in tissues such as the liver, kidney, skeletal muscle, cardiac muscle, and brown adipose tissue. The activity of SIRT3 is regulated by the availability of nutrients. Under fasting conditions, SIRT3 activates TCA cycle enzymes and promotes the switch from anabolic (glycolysis) to catabolic metabolism (β oxidation of fatty acids). SIRT3 also regulates inflammation, apoptosis, autophagy, and oxidative stress. Several studies have demonstrated a critical role of SIRT3 in several diseases such as Type 2 diabetes, aging, liver, kidney, inflammatory, cardiovascular, and neurodegenerative diseases. SIRT3 also plays a dual role in cancer; however, the role is tumor-specific. Due to its critical role in several human diseases, SIRT3 could serve as an efficient therapeutic target. Several small-molecule compounds are under investigation that target SIRT3 to treat human diseases. ’s SIRT3 (Human) ELISA kit quantitatively measures SIRT3 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of SIRT3 in the sample or standard.

Alternate Name : SIR2L3, SIRT3, Sirtuin 3, Sir2-Like 3, HSIRT3

Tag Line : A Sandwich ELISA kit for quantitative measurement of SIRT3 in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Serum Plasma Tissue lysate Other biological fluids

Species Reactivity : Human

Applications : Sandwich ELISA kit for quantitative measurement of SIRT3 in serum, plasma, cell and tissue lysates, and other biological fluids

Features and Benefits : Recovery range: 85 - 105% for normal human serum and plasma samples This Sandwich ELISA is highly sensitive and highly specific for the detection of SIRT3 in human samples. There is no significant cross-reactivity or interference between SIRT3 and analogues Detection range: 0.156 – 10 ng/ml Sensitivity: 0.094 ng/ml Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%