SIRT4 (GST-tagged), Human recombinant | 7673

There are seven human sirtuins, also known as class III HDACs, which have been designated SIRT1 to SIRT7. Each is involved in various post-translational modifications by utilizing NAD dependent deacetylase and APD-ribosyltransferase activities. SIRT4 is a mitochondrial ADP-ribosyltransferase responsible for the transfer of ADP-ribose from NAD to specific substrates such as glutamate dehydrogenase (GDH). In caloric-sufficient conditions, SIRT4 ADP-ribosylates GDH, repressing its activity and correspondingly decreasing insulin secretion. There is some uncertainty about the relevance of sirtuin ribosyl transferase activity due to its very slow rate, which is about 500 times lower than the corresponding deacetylation reaction (for those that have been characterized). SIRT4 is found in many tissues, but is specifically enriched in the kidney, heart, brain, and liver.

7673 | SIRT4 GST-tagged Human recombinant DataSheet

Biomolecule/Target: SIRT4

Synonyms: SIRT4 (GST-tagged), Human recombinant

Alternates names: IFN alpha II-1, IFNW1

Taglines: Intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

NCBI Gene ID #: 338376

NCBI Gene Symbol: IFNE1

Gene Source: Human

Accession #: Q86WN2

Recombinant: Yes

Source: E Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): The ED50 was determined by a cytotoxicity assay using Human TF-1 cells is 0.01 ng/ml, corresponding to a specific activity of 1 x 10^8 units/mg.

Biological activity: The ED50 was determined by a cytotoxicity assay using Human TF-1 cells is 0.01 ng/ml, corresponding to a specific activity of 1 x 10^8 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.9 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS