Ski Antibody | 2259

Ski Antibody | 2259 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (100%) , Horse: (100%) , Mouse: (85%)

Immunogen: Ski antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski.
The immunogen is located within the first 50 amino acids of Ski.

Research Area: Signal Transduction, Cancer

Tested Application: E, WB, IF

Application: SkiP antibody can be used for detection of Ski by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Ski Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Ski Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Ski antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Ski Antibody: SGS, SKV, Ski oncogene, Proto-oncogene c-Ski

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Ski Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.