SkiP Antibody | 2261

SkiP Antibody | 2261 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: SkiP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human SkiP.
The immunogen is located within the last 50 amino acids of SkiP.

Research Area: Signal Transduction

Tested Application: E, WB, IF

Application: SkiP antibody can be used for detection of SkiP by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate

Positive Control 2: Cat. No. 1305 - Human Kidney Tissue Lysate

Positive Control 3: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 58, 62 kDa
Observed: 65 kDa 

Validation: N/A

Isoform: N/A

Purification: SkiP Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SkiP Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: SkiP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SkiP Antibody: Bx42, SKIP, Prp45, SKIIP, PRPF45, NCOA-62, SNW domain-containing protein 1, Nuclear protein SkiP

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SkiP Antibody: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.