SLC1A7 Antibody | 8083

SLC1A7 Antibody | 8083 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: SLC1A7 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLC1A7.
The immunogen is located within amino acids 460 - 510 of SLC1A7.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: SLC1A7 antibody is human and mouse reactive. Multiple isoforms of SLC1A7 are known to exist.

Positive Control 1: Cat. No. 1285 - C2C12 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 17, 52, 62, 68 kDa
Observed: 52 kDa

Validation: N/A

Isoform: N/A

Purification: SLC1A7 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SLC1A7 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SLC1A7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Solute carrier family 1 (glutamate transporter) member 7, AAAT, excitatory amino acid transporter 5, EAAT5

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The solute carrier family 1 member 7 (SLC1A7) protein was initially identified as a glutamate transporter coupled to a chloride conductance that is expressed primarily in the retina (1) . More recent evidence indicates that is it widely expressed in peripheral tissues, also (2) . Experiments have shown that SLC1A7 functions as a low-affinity/low-capacity glutamate transport, with an anion channel optimized for anion conductance in the negative voltage range (3) . In the retina, SLC1A7 co-localizes with the serum and glucocorticoid dependent kinase SGK1, and its cell surface expression and activity is regulated SGK1 and its isoform SGK3 (4) .