Description
SLC29A1 Antibody | 8125 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Rat: (81%) , Mouse: (75%)
Immunogen: SLC29A1 antibody was raised against a 17 amino acid peptide near the center of human SLC29A1.
The immunogen is located within amino acids 240 - 290 of SLC29A1.
Research Area: Cancer, Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: SLC29A1 antibody can be used for detection of SLC29A1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: SLC29A1 antibody is human specific. SLC29A1 antibody is predicted to not cross-react with other SLC29 proteins.
Positive Control 1: Cat. No. 1316 - Human Ovary Tissue Lysate
Positive Control 2: Cat. No. 11-201 - Human Ovary Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 50 kDa
Observed: 53 kDa
Validation: N/A
Isoform: N/A
Purification: SLC29A1 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SLC29A1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SLC29A1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Solute carrier family 29 member 1, Equilibrative nucleoside transporter 1, ENT1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SLC29A1 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1, 2) . SLC29A1 is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium (3) . As a nucleoside transporter, SLC29A1 plays an important role in the uptake of nucleoside-based anti-cancer drugs; polymorphisms of point mutations in the gene encoding this protein may affect the efficacy of these drugs (4) .