SLC7A5 Antibody (N-Term) [APR33530G]

SLC7A5 Antibody (N-Term) [APR33530G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This SLC7A5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from human SLC7A5.

Cellular Localisation: Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Note=Located to the plasma membrane by SLC3A2/4F2hc (PubMed:9751058) . Localized to the apical membrane of placental syncytiotrophoblastic cells (PubMed:11742812) . Recruited to lysosomes by LAPTM4B (PubMed:25998567) . Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity) . {ECO:0000250, ECO:0000269|PubMed:11742812, ECO:0000269|PubMed:25998567, ECO:0000269|PubMed:9751058}

Molecular Weight: 55010

Clone: Polyclonal

Gene Name: SLC7A5

Gene ID: 8140

Function: The heterodimer with SLC3A2 functions as sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, L-DOPA, leucine, histidine, methionine and tryptophan (PubMed:9751058, PubMed:10049700, PubMed:11557028, PubMed:10391915, PubMed:10574970, PubMed:11311135, PubMed:11564694, PubMed:12117417, PubMed:12225859, PubMed:25998567, PubMed:30867591) . Functions as an amino acid exchanger (PubMed:11557028, PubMed:12117417, PubMed:12225859, PubMed:30867591) . May play a role in the transport of L-DOPA across the blood-brain barrier (By similarity) . May act as the major transporter of tyrosine in fibroblasts (Probable) . May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier (By similarity) . Can mediate the transport of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane (PubMed:11564694, PubMed:12225859) . When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation (PubMed:25998567) . Involved in the uptake of toxic methylmercury (MeHg) when administered as the L-cysteine or D, L-homocysteine complexes (PubMed:12117417) . Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the membrane (PubMed:15769744) .

Summary: Tissue Location: Detected in placenta, in the syncytiotrophoblast layer (at protein level) (PubMed:11389679) . Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid- trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, IF

Dilution: IF--1:25 WB--1:2000

Synonyms: Large neutral amino acids transporter small subunit 1, 4F2 light chain, 4F2 LC, 4F2LC, CD98 light chain, Integral membrane protein E16, L-type amino acid transporter 1, hLAT1, Solute carrier family 7 member 5, y+ system cationic amino acid transporter, SLC7A5, CD98LC, LAT1, MPE16