Additional Information
Size:  | 
                                                    100 µl  | 
                                                
Target:  | 
                                                    Smad3  | 
                                                
Conjugate:  | 
                                                    Dylight 405  | 
                                                
Research Area:  | 
                                                    Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic, Cancer, Cancer Metabolism, Response to Hypoxia  | 
                                                
Alternative Name:  | 
                                                    Smad3 Antibody, Mothers against decapentaplegic homolog 3 Antibody, Mad3 Antibody, SMAD3_HUMAN Antibody, DKFZp686J10186 Antibody, SMAD Antibody, HSPC193 Antibody, SMAD family member 3 Antibody, hMAD-3 Antibody, MGC60396 Antibody, MAD homolog 3 Antibo  | 
                                                
Category:  | 
                                                    Antibodies  | 
                                                
Product Type:  | 
                                                    Polyclonal  | 
                                                
Immunogen:  | 
                                                    A synthesized peptide derived from human Smad3  | 
                                                
Immunogen Species:  | 
                                                    Human  | 
                                                
Applications:  | 
                                                    WB, IHC  | 
                                                
Isotype:  | 
                                                    IgG  | 
                                                
Species Reactivity:  | 
                                                    Human, Mouse, Rat  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Peptide Affinity Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide  | 
                                                
Concentration:  | 
                                                    NA  | 
                                                
Specificity:  | 
                                                    Detects ~48kDa, endogenous levels of total Smad3  | 
                                                
Storage:  | 
                                                    -20ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    A 1:1000 dilution of SPC-1285 was sufficient for detection of Smad3 in 10 µg of Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.  | 
                                                
Cellular Localization:  | 
                                                    Cytoplasm, Nucleus  | 
                                                
Accession Number:  | 
                                                    NP_005893.1  | 
                                                
Gene ID:  | 
                                                    4088  | 
                                                
Swiss-Prot:  | 
                                                    P84022  | 
                                                
Tissue Specificity:  | 
                                                    NA  |