SMAD7 Antibody | 27-477

SMAD7 Antibody | 27-477 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD7.

Research Area: Transcription, Cancer, Signal Transduction

Tested Application: E, WB

Application: SMAD7 antibody can be used for detection of SMAD7 by ELISA at 1:12500. SMAD7 antibody can be used for detection of SMAD7 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

Specificiy: N/A

Positive Control 1: Cat. No. 1302 - Human Lung Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 46 kDa

Validation: N/A

Isoform: N/A

Purification: Antibody is purified by peptide affinity chromatography method.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Concentration: batch dependent

Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Alternate Name: SMAD7, CRCS3, MADH7, MADH8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition.