SMIF (S133) polyclonal Antibody | BS2145

SMIF (S133) polyclonal Antibody | BS2145 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: Signal transduction of TGF-β superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-β-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-β and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-β-induced nuclear translocation of SMIF (1) . The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SMIF (S133) polyclonal Antibody detects endogenous levels of SMIF protein.

Molecular Weight: ~ 75 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: mRNA-decapping enzyme 1A; Smad4-interacting transcriptional co-activator; Transcription factor SMIF; DCP1A; SMIF

Immunogen: Synthetic peptide, corresponding to amino acids 102-150 of Human SMIF.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: