SMPD1 Antibody (C-term) [APR07182G]

SMPD1 Antibody (C-term) [APR07182G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This SMPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-419 amino acids from the C-terminal region of human SMPD1.

Cellular Localisation: Lysosome. Lipid droplet. Secreted. Note=The secreted form is induced in a time- and dose-dependent by IL1B and TNF as well as stress and viral infection. This increase of the secreted form seems to be due to exocytosis of the lysosomal form and is Ca (2+) -dependent (PubMed:20807762, PubMed:22573858, PubMed:20530211) . Secretion is dependent of phosphorylation at Ser-510 (PubMed:17303575) . Secretion is induced by inflammatory mediators such as IL1B, IFNG or TNF as well as infection with bacteria and viruses (PubMed:12563314, PubMed:20807762)

Molecular Weight: 69936

Clone: Polyclonal

Gene Name: SMPD1 (HGNC:11120)

Gene ID: 6609

Function: Converts sphingomyelin to ceramide (PubMed:1840600, PubMed:18815062, PubMed:27659707, PubMed:25920558, PubMed:25339683, PubMed:33163980, PubMed:12563314) . Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly (PubMed:21098024, PubMed:9660788, PubMed:20807762) . However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form (PubMed:20530211, PubMed:12563314, PubMed:20807762, PubMed:9393854, PubMed:22573858) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P-Leica, WB, FC

Dilution: IHC-P-Leica--1:500 WB--1:4000

Synonyms: Sphingomyelin phosphodiesterase, Acid sphingomyelinase, aSMase, SMPD1, ASM