SMURF2 Antibody [AMM22248G]

SMURF2 Antibody [AMM22248G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: This SMURF2 antibody is generated from a mouse immunized with a recombinant protein of human SMURF2.

Cellular Localisation: Nucleus. Cytoplasm. Cell membrane. Membrane raft. Note=Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts

Molecular Weight: 86196

Clone: Monoclonal

Gene Name: SMURF2 (HGNC:16809)

Gene ID: 64750

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:11016919) . Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby downregulating TGF-beta signaling (PubMed:11163210, PubMed:12717440) . In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1 (PubMed:18448069) . Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation (PubMed:11016919, PubMed:11158580, PubMed:11389444) . SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation (PubMed:11389444) . Negatively regulates TGFB1-induced epithelial- mesenchymal transition and myofibroblast differentiation (PubMed:30696809) .

Summary: Tissue Location: Widely expressed.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000-1:4000

Synonyms: E3 ubiquitin-protein ligase SMURF2, hSMURF2, 6.3.2.-, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2