SMURF2 Antibody (C-term) [APR14407G]

SMURF2 Antibody (C-term) [APR14407G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, Rat

Specificity: This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 702-731 amino acids from the C-terminal region of human SMURF2.

Cellular Localisation: Nucleus. Cytoplasm. Cell membrane. Membrane raft. Note=Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts

Molecular Weight: 86196

Clone: Polyclonal

Gene Name: SMURF2 (HGNC:16809)

Gene ID: 64750

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:11016919) . Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby downregulating TGF-beta signaling (PubMed:11163210, PubMed:12717440) . In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1 (PubMed:18448069) . Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation (PubMed:11016919, PubMed:11158580, PubMed:11389444) . SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation (PubMed:11389444) . Negatively regulates TGFB1-induced epithelial- mesenchymal transition and myofibroblast differentiation (PubMed:30696809) .

Summary: Tissue Location: Widely expressed.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, IF

Dilution: WB--1:2000 WB--1:1000 IHC-P--1:100~500 IF--1:10~50

Synonyms: E3 ubiquitin-protein ligase SMURF2, hSMURF2, 632-, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2