SNAP25 Polyclonal Antibody | E-AB-40191

SNAP25 Polyclonal Antibody | E-AB-40191 | Gentaur UK, US & Europe Distribution

Type: Polyclonal Antibody

Synonyms: bA416N4.2, Bdr, CMS18, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC 9, SEC9, SNAP 25, SNAP, SNAP-25, SNAP-25B, SNAP25, SNP 25, SNP25, SNP25, sp, SUP, Super protein, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal associated protein 25, Synaptosomal associated protein 25kDa, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein 25, Synaptosomal-associated protein, 25-KD

Application: WB

Reactivity: Mouse

Host: Rabbit

Isotype: IgG

Reserch Areas: Cancer, Metabolism, Neuroscience, Signal Transduction

Background: The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by botulinum toxin E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.

Concentration: 2 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Recombinant Mouse Synaptosomal-associated protein 25 protein

Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Clone: N/A

Conjugation: Unconjugated

Molecular Weight(Calculated): 23 kDa

Molecular Weight(Observed): 23 kDa