Description
SPAG16 Antibody | 42-305 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen: The immunogen for this antibody is: C-KGVTELRTVGNVPD
Research Area: Other
Tested Application: E, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.8kDa according to NP_078808.3 and of 20.6kDa according to NP_001020607.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:This antibody was successfully used in Human and Murine frozen Brain sections (PMID: 25086173) .
Specificiy: This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1) .
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: SPAG16, sperm associated antigen 16, DKFZp666P1710, FLJ22724, FLJ37717, MGC87036, PF20, WDR29, WD repeat domain 29, sperm-associated WD repeat protein
User Note: N/A
BACKGROUND: N/A