SPATA1 Antibody | 6549

SPATA1 Antibody | 6549 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (72%)

Immunogen: SPATA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA1.
The immunogen is located within amino acids 300 - 350 of SPATA1.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: SPATA1 antibody can be used for detection of SPATA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of SPATA1 are known to exist; this antibody will detect only the longest isoforms. SPATA1 antibody is predicted to not cross-react with other SPATA family members.

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: SPATA1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SPATA1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SPATA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SPATA1 Antibody: SP-2, SPAP1, Spermatogenesis-associated protein 1, Sperm-specific protein SP-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SPATA1 Antibody: SPATA1, a novel cell-surface protein, is involved in shaping the sperm head during spermatogenesis. SPATA1 is localized at chromosome 1q21. At least three isoforms of SPATA1 are known to exist. The longest is preferentially expressed in hematopoietic tissues. Its extracellular domain contains a single immunoglobulin-like domain, and its intracellular segment has two immunoreceptor tyrosine-based inhibition motifs (ITIMs) . It is thought to have an important role in hematopoietic cell signaling. The mid-sized isoform contains a short intracellular part without ITIMs, while the shortest isoform lacks the transmembrane segment and represents a potential soluble protein (2, 3) .