Spred2 Antibody | 4849

Spred2 Antibody | 4849 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (85%) , Rat: (85%)

Immunogen: Spred2 antibody was raised against a 13 amino acid synthetic peptide near the center of the human Spred2.
The immunogen is located within amino acids 140 - 190 of Spred2.

Research Area: Signal Transduction

Tested Application: E, WB

Application: Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.

Positive Control 1: Cat. No. 1308 - Human Small Intestine Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Spred2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Spred2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Spred2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Spred2 Antibody: Spred-2, Sprouty-related, EVH1 domain-containing protein 2, Spred-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Spred2 Antibody: Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC) , suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. At least two isoforms of Spred2 are known to exist.