Sprouty Antibody | 46-430

Sprouty Antibody | 46-430 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse

Homology: N/A

Immunogen: The immunogen for this antibody is: CPSRGQGKPS

Research Area: Cancer

Tested Application: E, WB, IF, Flow, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ) . Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162) . Recommended concentration: 1-3ug/ml.Immunohistochemistry:This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Specificiy: NP_005832.1 and NP_955359.1 represent varients of the same protein.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: SPRY1, hSPRY1, sprouty homolog 1, antagonist of FGF signaling (Drosophila) , sprouty (Drosophila) homolog 1 (antagonist of FGF signaling) , sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)

User Note: N/A

BACKGROUND: N/A