SPT13 Antibody (Center) [APR05654G]

SPT13 Antibody (Center) [APR05654G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This SPT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the Central region of human SPT13.

Cellular Localisation: Cytoplasm. Cell projection, filopodium. Cell projection, lamellipodium. Cell projection, ruffle membrane Note=Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment

Molecular Weight: 74820

Clone: Polyclonal

Gene Name: SPATA13 {ECO:0000303|PubMed:28397838, ECO:0000312|HGNC:HGNC:23222}

Gene ID: 221178

Function: Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.

Summary: Tissue Location: Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, FC

Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50

Synonyms: Spermatogenesis-associated protein 13, APC-stimulated guanine nucleotide exchange factor 2, Asef2, SPATA13