SPTBN2 Antibody [SPTBN2/1583] | 33-752

SPTBN2 Antibody [SPTBN2/1583] | 33-752 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.

Research Area: Neuroscience, Signal Transduction

Tested Application: IHC-P, Flow, IF, WB

Application: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 0.5-1 ug/ml
IHC (FFPE) : 0.5-1 ug/ml for 30 min at RT
WB: 0.5-1 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: SPTBN2/1583

Isotype: IgG2b, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alphaII (fodrin alpha) and spectrin betaI (fodrin beta) are present in other somatic cells. SPTBN2/Spectrin betaIII is highly homologous to both spectrin betaI and spectrin betaII. SPTBN2 is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.