Sqstm1 (S351) Antibody [APR04661G]

Sqstm1 (S351) Antibody [APR04661G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This Phospho-Sqstm1 (S351) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 346-379 amino acids from the human region of human Phospho-Sqstm1 (S351) .

Cellular Localisation: Cytoplasm, cytosol. Late endosome. Nucleus Endoplasmic reticulum. Lysosome. Cytoplasmic vesicle, autophagosome Nucleus, PML body {ECO:0000250|UniProtKB:Q13501}. Cytoplasm, myofibril, sarcomere. Note=In cardiac muscles, localizes to the sarcomeric band (By similarity) . May also localize to the hepatocellular carcinoma (By similarity) . Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum (By similarity) . Commonly found in inclusion bodies containing polyubiquitinated protein aggregates (PubMed:20421418) . Co- localizes with TRIM5 in the cytoplasmic bodies (By similarity) . When nuclear export is blocked by treatment with leptomycin B, accumulates in PML bodies (By similarity) . {ECO:0000250|UniProtKB:Q13501, ECO:0000269|PubMed:20421418}

Molecular Weight: 48163

Clone: Polyclonal

Gene Name: Sqstm1

Gene ID: 18412

Function: Autophagy receptor required for selective macroautophagy (aggrephagy) . Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin- containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation (By similarity) . May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (PubMed:14960283, PubMed:18382763) . May be involved in cell differentiation, apoptosis, immune response and regulation of K (+) channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (By similarity) . Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes (By similarity) . Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR (KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes (PubMed:20421418, PubMed:20173742) .

Summary: Tissue Location: Widely expressed..

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Sequestosome-1, STONE14, Ubiquitin-binding protein p62, Sqstm1, A170, STAP