Additional Information
Size: |
100 µl |
Target: |
SRC (pTyr529) |
Conjugate: |
Biotin |
Research Area: |
Cell Signaling | Protein Phosphorylation | Tyrosine Kinases | Src Family |
Alternative Name: |
Neuronal CSRC tyrosine specific protein kinase Antibody, cSrc Antibody, Proto-oncogene c-Src Antibody, SRC Antibody, p60Src Antibody, c SRC Antibody, Tyrosine kinase pp60c src Antibody, pp60csrc Antibody, CDNA FLJ14219 fis clone NT2RP3003800 highly s |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
Synthetic phospho-peptide from human Src around the phosphorylation site of tyrosine 529 (pTyr529). |
Immunogen Species: |
Human |
Applications: |
WB, IHC, ELISA |
Host: |
Rabbit |
Isotype: |
IgG |
Species Reactivity: |
Human, Mouse, Rat |
Antibody Dilution: |
WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:5000); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide |
Concentration: |
N/A |
Specificity: |
Detects endogenous levels of Src only when phosphorylated at tyrosine 529 (pTyr529). |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:500 dilution of SPC-1429 was sufficient for detection of SRC (pTyr529) in 10 µg of human embryonic kidney epithelial cell line (hek293) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody. |
Cellular Localization: |
Mitochondrion, Mitochondrion Inner Membrane, Plasma Membrane, Cell Membrane, Cytoskeleton, Nucleus, Perinuclear Region, Actin Filament, Podosome, Cytosol, Endosome, Late Endosome, Extracellular Exosome, Lysosome, Nucleoplasm, Caveola, Extrinsic Compo |
Tissue Specificity: |
Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. |
Accession Number: |
NP_005408.1 |
Gene ID: |
6714 |
Swiss-Prot: |
P12931 |
Field of Use: |
For in vitro research use only. |