SREBF1 Antibody | 7853

SREBF1 Antibody | 7853 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: SREBF1 antibody was raised against a 17 amino acid peptide near the center of human SREBF1.
The immunogen is located within amino acids 450 - 500 of SREBF1.

Research Area: Obesity

Tested Application: E, WB, IHC-P, IF

Application: SREBF1 antibody can be used for detection of SREBF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: SREBF1 antibody is human and mouse reactive. At least three isoforms of SREBF1 are known to exist. SREBF1 antibody is predicted not to cross-react with SREBF2.

Positive Control 1: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 129 kDa
Observed: 132 kDa

Validation: N/A

Isoform: N/A

Purification: SREBF1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SREBF1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SREBF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SREBF1 Antibody: SREBP1, bHLHd1, SREBP-1c, BHLHD1, SREBP1, Sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, SREBP-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The sterol regulatory element binding transcription factor 1 (SREBF1) is a transcription factor that binds to the sterol regulatory element-1 (SRE1) , which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1) . The related protein SREBF2 also binds SRE1 and activates transcription in an additive fashion to SREBF1 (2) . SREBF1 is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1 (3) . The SREBF1 proteins are important in the regulation of genes involved in lipid metabolism, while SREBF2 has been more closely associated with cholesterol synthesis and accumulation (4) .