SREBF2 Antibody | 7855

SREBF2 Antibody | 7855 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (100%)

Immunogen: SREBF2 antibody was raised against a 15 amino acid peptide near the center of human SREBF2.
The immunogen is located within amino acids 440 - 490 of SREBF2.

Research Area: Obesity

Tested Application: E, WB, IHC-P, IF

Application: SREBF2 antibody can be used for detection of SREBF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: SREBF2 antibody is human and mouse reactive. At least three isoforms of SREBF2 are known to exist. SREBF2 antibody is predicted not to cross-react with SREBF1.

Positive Control 1: Cat. No. 1216 - PC-3 Cell Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 126 kDa
Observed: 128 kDa

Validation: N/A

Isoform: N/A

Purification: SREBF2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SREBF2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SREBF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SREBF2 Antibody: SREBP2, bHLHd2, SREBP-2, BHLHD2, SREBP2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The sterol regulatory element binding transcription factor 2 (SREBF2) is a transcription factor that binds to the sterol regulatory element-1 (SRE1) , which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1, 2) . The related protein SREBF1 also binds SRE1 and activates transcription in an additive fashion to SREBF2 (2) . SREBF2 has been more closely associated with cholesterol synthesis and accumulation, while the SREBF1 proteins are important in the regulation of genes involved in lipid metabolism (3) .