SRPK1 Antibody | 8435

SRPK1 Antibody | 8435 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: SRPK1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human SRPK1.
The immunogen is located within the first 50 amino acids of SRPK1.

Research Area: Cell Cycle, Homeostasis

Tested Application: E, WB, IHC-P

Application: SRPK1 antibody can be used for the detection of SRPK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 5 μg/ml.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2.

Positive Control 1: Cat. No. 1404 - Mouse Liver Tissue Lysate

Positive Control 2: Cat. No. 11-701 - Human Testis Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 54, 72, 91 kDa
Observed: 91 kDa

Validation: N/A

Isoform: N/A

Purification: SRPK1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SRPK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SRPK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SRSF protein kinase 1, Serine/arginine-rich protein-specific kinase 1, Serine/threonine-protein kinase SRPK1, SFRSK1, SR-protein-specific kinase 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SRPK1 and SRPK2, two Arginine/serine-rich (SR) protein-specific kinases constitute control circuits to regulate both constitutive and alternative pre-mRNA splicing and coordinate splicing with transcription in cells (1) . They are highly specific for the phosphorylation of these RS proteins, and highly expressed in testis (1, 2) . SRPK1 plays a role in the condensation of sperm chromatin and controls the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis (3, 4) .