ST3Gal1, soluble fragment, Human Recombinant | P1224

ST3Gal1 catalyzes transfer of the sialic acid Neu5Ac from CMP-Neu5Ac (cytidine 5'-monophosphono-N-acetylneuraminic acid) to Gal1, 3GalNAc on glycoproteins or glycolipids in an 2, 3 linkage. It is a type II transmembrane protein that is normally localized to the golgi, but can be cleaved to yield a soluble product. Sialylation of the core-1 structure (Gal1, 3GalNAc-O-Ser/Thr) by ST3Gal1 prevents formation of the core-2 structure (GlcNAc1, 6(Gal1, 3)GalNAc-Ser/Thr). On CD8+ T-cells this prevents induction of either apoptosis or differentiation into memory cells. Overexpression of ST3Gal1 in breast cancer cells correlated with the presence of the core-1 based marker SM3, whereas healthy cells displayed a higher ratio of core-2 based epitopes. Overexpression of ST3Gal1 in colorectal carcinoma was found to be related to poor patient survival.

P1224 | ST3Gal1 soluble fragment Human Recombinant DataSheet

Biomolecule/Target: ST3Gal1

Synonyms: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1, Gal-NAc6S, Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase, SIATFL, ST3Gal I

Alternates names: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1, Gal-NAc6S, Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase, SIATFL, ST3Gal I

Taglines: Involved in the pathway protein glycosylation, which is part of Protein modification

NCBI Gene ID #: 28106

NCBI Gene Symbol: CS010

Gene Source: Human

Accession #: Q9CPT4

Recombinant: Yes

Source: Insect cells

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 45 kDa

Concentration: 100 ug/ml

Appearance: Liquid

Physical form description: Sterile filtered solution in 25 mM Tris pH 7.5 and 150 mM NaCl

Reconstitution Instructions: Reconstitute in sterile ddHO to a concentration 100 µg/ml. This solution can then be diluted into other aqueous buffers.

Amino acid sequence: MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL