171

Stable GS-3A4-HepG2 Cell Line | T6016

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SKU:
171-T6016-GEN
Availability:
IN STOCK
NULL4,173.00

Description

Stable GS-3A4-HepG2 Cell Line | T6016

The parental cell line of GS-3A4-HepG2, HepG2, was derived from a human hepatoma and was transformed with a vector containing the Glutamine Synthetase gene, allowing for ammonia metabolism and the 3A4 gene for drug metabolism. This transformation allows for activity in the immortalized cell line that is similar to primary hepatocytes, but with a greater ability to metabolise drugs (3A4 activity). This cell line is highly functional in long term culture, and therefore ideal for continuous research into liver function and metabolism. Previous studies have explored the cell line’s use into bioartificial liver support systems. These systems are new technologies being developed to treat cases of liver damage where function needs to be supported during liver regeneration. The GS-3A4-HepG2 cell line may be particularly useful to metabolism drugs during long term experiments.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

13 - 23 hours

Seeding Density:

20,000 - 40,000 cells/cm2

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

Male

Donor Ethnicity:

Caucasian

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoatTM T25 Flasks

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C

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Additional Information

Size:
10⁶ cells/1.0 ml
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