STAT1 Antibody [1141CT26.2.1] | 64-233

STAT1 Antibody [1141CT26.2.1] | 64-233 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Purified His-tagged STAT1 protein was used to produced this monoclonal antibody.

Research Area: Cancer, Obesity, Neuroscience, Signal Transduction

Tested Application: WB

Application: For WB starting dilution is: 1:1000

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 87 kDa

Validation: N/A

Isoform: N/A

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Clonality: Monoclonal

Clone: 1141CT26.2.1

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration: batch dependent

Storage Condition: Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs) , cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426) . ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG) , which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma) , STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788) . It then forms a homodimer termed IFN-gamma-activated factor (GAF) , migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.