Additional Information
Size:  | 
                                                    100 µl  | 
                                                
Target:  | 
                                                    STAT3 (pSer727)  | 
                                                
Conjugate:  | 
                                                    PE/ATTO 594  | 
                                                
Research Area:  | 
                                                    Cell Signaling | Epigenetics and Nuclear Signaling | Nuclear Signaling Pathways | STATs | Cell Signaling | Epigenetics and Nuclear Signaling | Transcription | Cancer | Stem Cells | Embryonic Stem Cells | ESC Intracellular Markers | Cardiovascular Sys  | 
                                                
Alternative Name:  | 
                                                    STAT3 Antibody, MGC16063 Antibody, ADMIO Antibody, 1110034C02Rik Antibody, Acute-phase response factor Antibody, HIES Antibody, STAT 3 Antibody, Signal transducer and activator of transcription 3 (acute phase response factor) Antibody, AW109958 Antib  | 
                                                
Category:  | 
                                                    Antibodies  | 
                                                
Product Type:  | 
                                                    Polyclonal Antibody  | 
                                                
Immunogen:  | 
                                                    Synthetic phospho-peptide from human STAT3 around the phosphorylation site of serine 727 (pSer727).  | 
                                                
Immunogen Species:  | 
                                                    Human  | 
                                                
Applications:  | 
                                                    WB, IHC, ELISA, IP  | 
                                                
Host:  | 
                                                    Rabbit  | 
                                                
Isotype:  | 
                                                    IgG  | 
                                                
Species Reactivity:  | 
                                                    Human, Mouse, Rat  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:5000); IP (Various dilutionss); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Peptide Affinity Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide  | 
                                                
Concentration:  | 
                                                    N/A  | 
                                                
Specificity:  | 
                                                    Detects endogenous levels of STAT3 only when phosphorylated at serine 727 (pSer727).  | 
                                                
Storage:  | 
                                                    -20ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    A 1:500 dilution of SPC-1431 was sufficient for detection of STAT3 (pSer727) in 10 µg of human cervical cancer cell line (hela) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.  | 
                                                
Cellular Localization:  | 
                                                    Nucleus, Cytoplasm, Cytosol, Mitochondrion, Mitochondrion Inner Membrane, Nuclear Chromatin, Nucleoplasm, RNA Polymerase II Transcription Factor Complex  | 
                                                
Tissue Specificity:  | 
                                                    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.  | 
                                                
Accession Number:  | 
                                                    NP_003141.2  | 
                                                
Gene ID:  | 
                                                    6774  | 
                                                
Swiss-Prot:  | 
                                                    P40763  | 
                                                
Field of Use:  | 
                                                    For in vitro research use only.  |