STK3, Active | 7751

Creasy identified a cDNA encoding STK3, which they called MST2. Sequence analysis indicated that the deduced 491-amino acid STK3 protein contains an N-terminal catalytic domain characteristic of STKs (1). Taylor also isolated cDNAs encoding STK3 (p63) and STK4 (p61) respectively. STK3 and STK4 share 94% amino acid identity in the catalytic domain and 78% identity overall (2). The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. O'Neill used proteomic analysis of RAF1 signaling complexes to show that RAF1 counteracts apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2) (3). MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (2; 4). STK3 activation presumably allows cells to resist unfavorable environmental conditions.

7751 | STK3 Active DataSheet

Biomolecule/Target: N/A

Synonyms: STK, serine/threonine kinase 3

Alternates names: STK, serine/threonine kinase 3, Mammalian STE20-like protein kinase 2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1

Taglines: Allows cells to resist unfavorable environmental conditions.

NCBI Gene ID #: 7042

NCBI Gene Symbol: TGFB2

Gene Source: N/A

Accession #: P61812

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 277 nmol phosphate incorporated into MBP per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED as determined by the ability of TGF-2 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is <0.2 ng/ml, corresponding to a specific activity of > 5 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 87.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in 1 mM Citrate buffer (pH 3.5) to a concentration of 100-1000 ug/ml. The solution can then be stored at 4°C for 1 month. It is recommended that further dilutions be made into PBS containing 2 mg/ml albumin and stored at –20°C.

Amino acid sequence: N/A