STN8 | Serine/threonine-protein kinase STN8 (chloroplastic) | AS10 1601

STN8 | Serine/threonine-protein kinase STN8 (chloroplastic) | AS10 1601 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conugated synthetic peptide (amino acids 425 - 438) specific for Arabidopsis thaliana STN8 serine/threonine proteinkinase, UniProt: Q9LZV4 TAIR:At5g01920

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS15 3080 | Anti-STN7 | serine/threonine-protein kinase STN7, chloroplastic for Chlamydomonas reinhardtii, rabbit antibodiesSecondary antibodies

Recommended Dilutions: 1 : 2000 (WB)

Molecular weight: 54, 9 kDa or 46 kDa on 6 M urea gel

Confirmed Reactivity: Arabidopsis thaliana, Triticum aestivum

Predicted Reactivity: Oryza sativa Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: For best results with this antibody sample buffer needs to contain 6 M urea (138mM TrisHCl pH 6.8, 6M urea, 22.2% Glycerol, 4.3% SDS) .STN8 is a nuclear encoded protein which is localized in chloroplast, hence posses a chloroplastic target peptides (cTP) at the beginning of the amino acid sequence which is cut off. Accroding to TargetP (program that predicts the length of the cTP:s) the length of cTP for Stn8 is 49 amino acids. STN8 portein mobility can be also affected by urea present in the gel.Due to a high background signal with LHCII it is adviced to cut off a membrane below 30 kDa marker.

Background: Serine/threonine protein kinase (EC=2.7.11.1), known as STN8 in Arabidopsis, and its Stl1 homologue in Chlamydomonas, are STN7/Stt7-like proteins that are not required for state transitions. The Arabidopsis mutants deficient in stn8 gene demonstrated highly reduced levels of the phosphorylated proteins of the photosystem II (Bonardi et al., 2005 Varionen et al., 2005) . Alternative name: Protein STATE TRANSITION 8.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G01920

Category: Kinases

Research Area: Plant Biology