SUFU Antibody [AMM02579G]

SUFU Antibody [AMM02579G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H, M, African Green Monkey

Specificity: This SUFU antibody is generated from a mouse immunized with a recombinant protein of human SUFU.

Cellular Localisation: Cytoplasm. Nucleus

Molecular Weight: 53947

Clone: Monoclonal

Gene Name: SUFU {ECO:0000303|PubMed:12068298, ECO:0000312|HGNC:HGNC:16466}

Gene ID: 51684

Function: Negative regulator in the hedgehog/smoothened signaling pathway (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:12068298, PubMed:12975309, PubMed:27234298, PubMed:15367681, PubMed:22365972, PubMed:24217340, PubMed:24311597, PubMed:28965847) . Down-regulates GLI1-mediated transactivation of target genes (PubMed:15367681, PubMed:24217340, PubMed:24311597) . Down-regulates GLI2-mediated transactivation of target genes (PubMed:24311597, PubMed:24217340) . Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:24217340) . Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:24217340) . Negative regulator of beta-catenin signaling (By similarity) . Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL) (PubMed:24311597, PubMed:28965847) . GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state (PubMed:24311597, PubMed:28965847) . Without the Hh signal, the SUFU- GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R (PubMed:24311597, PubMed:28965847) . When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A) (PubMed:24311597, PubMed:28965847) . Required for normal embryonic development (By similarity) . Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity) .

Summary: Tissue Location: Ubiquitous in adult tissues. Detected in osteoblasts of the perichondrium in the developing limb of 12-week old embryos. Isoform 1 is detected in fetal brain, lung, kidney and testis Isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, IHC-P

Dilution: IHC-P--1:100~500 WB--1:2000

Synonyms: Suppressor of fused homolog, SUFUH, SUFU