Superoxide Dismutase 2 (SOD-2), human recombinant | 6360

SOD2 is part of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. SOD2 binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in SOD2 gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. SOD2 destroys radicals which are usually produced within the cells and which are toxic to biological systems.

6360 | Superoxide Dismutase 2 SOD-2 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant SOD2

Alternates names: Superoxide dismutase [Mn], mitochondrial, IPOB, MNSOD, MVCD6.

Taglines: Enzyme that catalyze the dismutation of superoxide

NCBI Gene ID #: 7040

NCBI Gene Symbol: TGFB1

Gene Source: Human

Accession #: P01137

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the ability of TGF-1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is <0.05 ng/ml, corresponding to a specific activity of >2 x 10 units/mg.

Results: >1,200 units/mg

Binding Capacity: N/A

Unit Definition: One unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and xanthine oxidase at pH 7.8 at 25°C in a 1.5 ml reaction volume.

Molecular Weight: 24.4 kDa (219 aa, 25-222 aa + His Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 20% glycerol.

Reconstitution Instructions: Reconstitution in HO to a concentration of 50 ug/ml (e.g., 5 ug/100 l) will yield a solution of 1 mM citrate buffer (pH 3.5). The solution can then be stored at 4°C for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at –20°C.

Amino acid sequence: N/A