Survivin Antibody | 2235

Survivin Antibody | 2235 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Pig: (92%) , Bovine: (79%) , Mouse: (71%) , Rat: (71%)

Immunogen: Anti-Survivin antibody (2235) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Survivin.
The immunogen is located within the last 50 amino acids of Survivin.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, ICC, IF

Application: WB: 1-5 μg/mL; ICC: 10 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1206 - MOLT4 Cell Lysate

Positive Control 2: Cat. No. 17-006 - MOLT-4 Cell Slide

Positive Control 3: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 4: Cat. No. 1204 - K562 Cell Lysate

Positive Control 5: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 6: N/A

Molecular Weight: Predicted: 17kD
Observed: 17 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar Survivin expression profile in human cell lines detected by two independent anti-Survivin antibodies that recognize different epitopes, 2233 against N-terminus domain and 2235 against C-terminus domain.  Survivin proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Recombinant Protein Test (Figure 6- 8) : Anti-Survivin antibodies (2235) detected human Survivin recombinant protein directly or in sera from cancer patients.

Isoform: Human Survivin has 7 isoforms, including isoform 1 (142aa, 16kD) , isoform 2 (165aa, 19kD) , isoform 3 (137aa, 16kD) , isoform 4 (120aa, 14kD) , isoform 5 (117aa, 14kD) , isoform 6 (78aa, 9kD) and isoform 7 (74aa, 8.5kD) . Mouse Survivin has 3 isoforms, including isoform 1 (140aa, 16kD) , isoform 2 (121aa, 14kD) and isoform 3 (40aa, 4.7kD) . Rat Survivin has one isoform (142aa, 17kD) . 2235 can detect human and possibly mouse and rat.

Purification: Survivin Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Survivin Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Survivin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Survivin Antibody: API4, EPR-1, API4, IAP4, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Survivin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4) , and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.