T4 DNA Ligase (5 u/ l) | 9101

T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor.

9101 | T4 DNA Ligase 5 u/ l DataSheet

Biomolecule/Target: N/A

Synonyms: DNA Ligase

Alternates names: T4 DNA Ligase

Taglines: An enzyme which anneals DNA strands together

NCBI Gene ID #: 7040

NCBI Gene Symbol: TGFB1

Gene Source: N/A

Accession #: P01137

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the ability of TGF-1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is <0.05 ng/ml, corresponding to a specific activity of >2 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of T4 DNA ligase is the amount of enzyme activity required to ligate EcoR I digested 1.0 µg of plasmid DNA in 5 minutes at 20°C in a 20 µl Reaction.

Molecular Weight: N/A

Concentration: 5 U/µl

Appearance: Liquid

Physical form description: ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50% glycerol (v/v).

Reconstitution Instructions: Reconstitution in HO to a concentration of 50 ug/ml (e.g., 5 ug/100 l) will yield a solution of 1 mM citrate buffer (pH 3.5). The solution can then be stored at 4°C for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at –20°C.

Amino acid sequence: N/A