TACE (TNF- converting enzyme), human recombinant | 7607

TACE (TNF-alpha converting enzyme, ADAM17) is a membrane-bound Metalloprotease from the ADAM (a disintegrin and Metalloprotease) family of proteins. It cleaves 26kDa type II membrane-bound precursor of TNF-a into 17 kDa mature form which is secreted. TACE is also known to process other membrane proteins including TNF receptor, TGF-alpha and amyloid protein precursor. Recombinant active untagged TACE amino acid sequence is from Arg215 to Asn651. The apparent molecular weight is bigger than the calculated MW due to the protein posttranslational modification in eukaryotic system.

7607 | TACE TNF- converting enzyme human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, CD156b

Alternates names: Macrophage Inflammatory Protein-3, CCL23, MPIF-1, Ck-8

Taglines: Involved in the processing of TNF- at the surface of cells

NCBI Gene ID #: 6368

NCBI Gene Symbol: CCL23

Gene Source: Human

Accession #: P55773

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to chemoattract human T cells using a concentration range of 10.0-50.0 ng/ml.

Biological activity: Determined by its ability to chemoattract human T cells using a concentration range of 10.0-50.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 11.3 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN