TAF9 Antibody | 7719

TAF9 Antibody | 7719 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: TAF9 antibody was raised against a 17 amino acid peptide near the center of human TAF9.
The immunogen is located within amino acids 110 - 160 of TAF9.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members.

Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 29 kDa
Observed: 28 kDa

Validation: N/A

Isoform: N/A

Purification: TAF9 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TAF9 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TAF9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: TAF9 Antibody: TAF2G, TAFII31, TAFII32, MGC:5067, TAFII-31, TAFII-32, TAFIID32, STAF31/32, TAF2G, Transcription initiation factor TFIID subunit 9, RNA polymerase II TBP-associated factor subunit G

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Initiation of transcription by RNA polymerase II is coordinated by the transcription factor IID (TFIID) , which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and the TBP-associated factors (TAFs) . TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation (1) . TAF9 is one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16 (2) .