TARDBP Antibody (N-term) [APG02984G]

TARDBP Antibody (N-term) [APG02984G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M, Rat

Specificity: This TARDBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TARDBP.

Cellular Localisation: Nucleus. Cytoplasm. Cytoplasm, Stress granule Note=Continuously travels in and out of the nucleus (PubMed:18957508) Localizes to stress granules in response to oxidative stress (PubMed:19765185) .

Molecular Weight: 44740

Clone: Polyclonal

Gene Name: TARDBP

Gene ID: 23435

Function: RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed:23519609) . Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed:23519609, PubMed:24240615, PubMed:24464995) . In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed:21358640, PubMed:29438978) . Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed:28794432) . Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly (A) tail deadenylation and thus shortening (PubMed:30520513) . In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed:23398327, PubMed:19765185) . Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed:30464263) . Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed:27123980) . Negatively regulates the expression of CDK6 (PubMed:19760257) . Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed:25678563) .

Summary: Tissue Location: Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, IF, WB

Dilution: IHC-P--1:100 WB--1:2000

Synonyms: TAR DNA-binding protein 43, TDP-43, TARDBP, TDP43