TDGF1, human recombinant | 4335

Teratocarcinoma-derived growth factor 1, also known as epidermal growth factor-like cripto protein CR1, CRGF, and TDGF1, is a cell membrane which contains one EGF-like domain. In humans, TDGF1 is highly expressed in germ cell tumors and in colon and mammary carcinomas. TDGF1 is a member of the epidermal growth factor-cripto FRL1 cryptic protein family and is involved in the activation of several different signaling pathways during embryonic development and cellular transformation. TDGF1 regulates the activation of several signaling pathways and controls cellular transformation in embryonic status. Patients with high TDGF1 expression were statistically susceptible to a recurrence of the disease, and showed poorer disease-free survival than those with low expression. TDGF1 is a predictive marker for metachronous metastasis in colorectal cancer (CRC) patients. It is preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. TDGF1 plays a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

4335 | TDGF1 human recombinant DataSheet

Biomolecule/Target: TDGF1

Synonyms: CRIPTO1, CRIPTO-1, CRGF, Teratocarcinoma-Derived Growth Factor 1, TDGF1, CR1.

Alternates names: Nesfatin 1

Taglines: A signaling protein involved in embryonic development and tumor growth

NCBI Gene ID #: 4925

NCBI Gene Symbol: NEFA

Gene Source: Human

Accession #: P80303

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by an in vivo assay using healthy wild type male mice (C57BL/6J). Mice were treated via intraperitoneal injection once at a dose of 4g Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.

Biological activity: Determined by an in vivo assay using healthy wild type male mice (C57BL/6J). Mice were treated via intraperitoneal injection once at a dose of 4g Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 9.7 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.1% TFA

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL