TDP43 Antibody | 4285

TDP43 Antibody | 4285 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (94%)

Immunogen: TDP43 antibody was raised against a 18 amino acid synthetic peptide from near the center of human TDP43.
The immunogen is located within amino acids 160 - 210 of TDP43.

Research Area: Neuroscience

Tested Application: E, WB, ICC, IF

Application: TDP43 antibody can be used for detection of TDP43 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms are known to exist for this protein; this TDP43 antibody only recongizes the longer of the two isoforms.

Positive Control 1: Cat. No. 1284 - L1210 Cell Lysate

Positive Control 2: Cat. No. 17-001 - HeLa Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 46 kDa
Observed: 46 kDa

Validation: N/A

Isoform: N/A

Purification: TDP43 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TDP43 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TDP43 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TDP43 Antibody: ALS10, TDP-43, TDP43, TAR DNA-binding protein 43

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TDP43 Antibody: TDP43 was initially identified as a novel cellular protein that bound to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR) , most likely through the association with the UG repeats at the 3'splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U) . TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.