TEM1 Antibody | 4357

TEM1 Antibody | 4357 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse (86%) ; Rat: (86%)

Immunogen: Anti-TEM-1 antibody (4357) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1.
The immunogen is located within the last 50 amino acids of TEM-1.

Research Area: Homeostasis, Cancer

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5-2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.

Positive Control 1: Cat. No. 1320 - Human Colon Tissue Lysate

Positive Control 2: Cat. No. 1223 - Caco-2 Cell Lysate

Positive Control 3: Cat. No. 1219 – MCF-7 Cell Lysate

Positive Control 4: Cat. No. 1220 – SK-N-SH Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 81kD and 46kD
Observed: 90 kD (post-modifications: 27 O-linked glycosylations) and 46kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TEM-1 expression profile in human and mouse cell lines detected by two independent anti-TEM-1 antibodies that recognize different epitopes, 4357 against C-terminus domain and 4359 against N-terminus domain.  TEM-1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Isoform: Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD) and isoform 2 (433aa, 46kD) . Mouse TEM-1 has one isoform (765aa, 82kD) and rat TEM-1 also has one isoform (764aa, 82kD) . 4357 can detect all isoforms of human, mouse and rat.

Purification: TEM1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TEM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TEM1 Antibody: TEM1, CD164L1, CD248, Endosialin, Tumor endothelial marker 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.