Description
TESPA1 Antibody | 7601 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen: TESPA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human TESPA1.
The immunogen is located within amino acids 20 - 70 of TESPA1.
Research Area: Immunology
Tested Application: E, WB, ICC, IF
Application: TESPA1 antibody can be used for detection of TESPA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: TESPA1 antibody is human specific. At least three isoforms TESPA1 are known to exist.
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: Cat. No. 17-002 - A-431 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 57 kDa
Observed: 60 kDa
Validation: N/A
Isoform: N/A
Purification: TESPA1 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TESPA1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TESPA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: TESPA1 Antibody: HSPC257, KIAA0748, HSPC257, Protein TESPA1, Thymocyte-expressed positive selection-associated protein 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1) . TESPA1 encodes a protein of 458 amino acids and is highly conserved among vertebrate species (2) . TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3) . TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1, 4) .