TfR mutant protein:A529G, recombinant (hFc Tag) | NCP0125

TfR mutant protein:A529G, recombinant (hFc Tag) | NCP0125 | Gentaur UK, US & Europe Distribution

Host: Human

Reactivity: 293F

Application: The protein has a calculated MW of 76 kDa.

Background: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes . Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity) . A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2.

Concentration: PBS, pH7.4

Purification: The protein was purified from 293F and the purity is > 95% (by SDS-PAGE) .

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: AA Leu 122 - Ile 550 (Accession # P02786)

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: N/A

Immunogen: N/A