TGF-beta1, human recombinant | 4342

TGF-1 (Transforming Growth Factor-1) is a member of the superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF-1 is a 25.0 kDa protein containing two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

4342 | TGF-beta1 human recombinant DataSheet

Biomolecule/Target: TGF-beta 1

Synonyms: Transforming Growth Factor-1, Differentiation inhibiting factor, Cartilage-inducing factor

Alternates names: OSM

Taglines: A protein that controls proliferation, cellular differentiation, & other functions in most cells

NCBI Gene ID #: 289747

NCBI Gene Symbol: Osm

Gene Source: Rat

Accession #: Q65Z15

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Assay #1: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 3.0-5.0 g/ml. Assay #2: Determined by its ability to inhibit Alkaline phosphatase activity of differentiated MC3T3 E1 cells.

Biological activity: Assay #1: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 3.0-5.0 g/ml. Assay #2: Determined by its ability to inhibit Alkaline phosphatase activity of differentiated MC3T3 E1 cells.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 24.4 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP SRSSQSAMLR SLVPR