Description
TIF1 alpha Antibody | 46-493 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Cow
Immunogen: The immunogen for this antibody is: C-KRLKSIEERQLLK
Research Area: Cancer
Tested Application: E, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 120kDa band in human kidney and heart lysates at 1ug/ml, however prominent bands were also seen at 25kDa and 45kDa. Please note that currently we cannot find an explanation in the literature for some of the bands we observe given the predicted sizes of approx. 117kDa and 121kDa according to NP_003843 and NP_056989. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Breast cancer shows nuclear staining in the distorted lobules. Recommended concentration, 4-6ug/ml.
Specificiy: This antibody is expected to recognise both reported isoforms, as represented by NP_003843.3 and NP_056989.2.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: TRIM24, TIF1, transcriptional intermediary factor 1, PTC6, TF1A, TIF1A, Tif1a, hTIF1, TIF1ALPHA, transcriptional intermediary factor 1 alpha, tripartite motif protein 24, tripartite motif-containing 24, RNF82
User Note: N/A
BACKGROUND: N/A